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The significant common peaks were labeled as mark peaks and the reference. This study suggests not only that many of the drugs repurposed for SARS-CoV-2 in biochemical assays are artifacts but that, more generally, at screening-relevant concentrations, even drugs can act artifactually via colloidal aggregation. The data were recorded and processed using the Agilent Chemstation software. From a common repurposing library, we further selected another 15 drugs that had physical properties resembling known aggregators, finding that six aggregated at micromolar concentrations. ChemStation B.03 Printed in April, 2007 Agilent 1100/1200 HPLC ChemStation Operation Course Number H4033A Laboratory Manual. It is a scalable data system ideally suited for applications in all industries ranging from early product development to quality control. Agilent 1100/1200 HPLC ChemStation Operation Course Number H4033A Laboratory Manual. We tested 56 drugs reported as active in biochemical assays for aggregation by dynamic light scattering and by detergent-based enzyme counter screening 19 formed colloids at concentrations similar to their literature IC 50’s, and another 14 were problematic. Agilent ChemStation is the industry leading chromatography data system for Agilent instrumentation, handling the widest variety of separation techniques such as GC, LC, LC/MS, CE and CE/MS.
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Here, we investigate the impact of colloidal aggregation, a common screening artifact, in these repurposing campaigns.
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#AGILENT CHEMSTATION USER MANUAL MANUALS#
Using the manual on CD-ROM 1 The manuals on the CD are presented in Adobe Acrobat PDF format.
#AGILENT CHEMSTATION USER MANUAL HOW TO#
To fight COVID-19, much effort has been directed toward in vitro drug repurposing. GC/MSD ChemStation Getting Started 11 Hardware manuals on CD-ROM Each piece of hardware is accompanied by a CD-ROM which contains hundreds of pages of reference material as well as videos describing how to operate, maintain, and troubleshoot the equipment.